Vitamin Analysis in Food

Vitamin analysis is carried out either microbiologically using the  VitaFast® kits developed by ifp or by means of instrumental equipment (high performance liquid chromatography, HPLC).

Determination of water-soluble vitamins using VitaFast®

Analysis of all water-soluble vitamins (except for vitamin C) is done with the VitaFast® kits developed and produced by ifp. These kits are an innovative advancement of the official method which has been the gold standard in the analysis of water-soluble vitamins for decades. The method uses microorganisms, the growth of which depends on the presence of the vitamin in the sample, in order to detect and quantify the vitamin of interest.

This traditional method requires a substantial amount of work and extensive microbiological expertise. The time to result averages one week or more.

With VitaFast®, ifp successfully transferred the official method into a ready-to-use microtiter plate format. Thus, incoming samples can be analysed immediately and with high precision. Results are usually available after just 48 hours.

The vitamin C (ascorbic acid) content in foodstuffs is determined by means of a microtiter plate-based enzymatic assay. Unlike the conventional method, this format no longer requires single measurements in cuvettes, thus allowing for much more efficient sample processing.

Quantification limits for water-soluble vitamins in 100 g (ml)1

  • B1 (thiamine): 0.012 mg
  • B2 (riboflavin): 0.04 mg
  • B3 (niacin): 0.016 mg
  • B5 (pantothenic acid): 0.04 mg
  • B6 (pyridoxine): 0.002 mg
  • B7 (biotin): 0.08 µg
  • B9 (folic acid): 0.16 µg
  • B12 (cyanocobalamin): 0.03 µg
  • Inositol: 0.5 mg
  • C (ascorbic acid): < 5 mg

1 For a 1 g sample; the quantification limit can be further decreased by    increasing the amount of sample.

Quantification limits for fat-soluble vitamins and Vitamin C using HPLC

The fat-soluble parameters and vitamin C are determined by high performance liquid chromatography (HPLC). Detection is carried out using a fluorescence detector (FLD) or a diode array UV detector (DAD).

  • A (β-carotene/retinol): 4.0 µg1
  • D2/D3 (ergocalciferol/cholecalciferol): 0.3 µg1
  • E (α-*, β-, δ-, ε-tocopherols): 0.2 µg1
  • K1/K2 (phylloquinone/menaquinone): 2.0 µg2
  • C (ascorbic acid): 0.5 mg3

based on a sample amount of: 1 10 g; 2 3 g; 3 5 g
* enantiomer separation possible for α-D- and α-L-tocopherol